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. 2013 Mar 15;24(6):692–703. doi: 10.1091/mbc.E12-10-0719

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© 2013 Marada et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Circular dichroism analysis of Mge1 under oxidative stress. Purified and dialyzed Mge1 (40 μM) wild-type and Mge1-M155L mutant samples in phosphate buffer were used for CD spectra analysis at 20°C at a scan rate of 60°C/h in a 2-mm–path-length cuvette_. Wild type (A) and mutant Mge1 (B) were pretreated with 0.5, 1, or 2 mM H₂O₂ for 15 min before spectral analysis.