Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Mar 15;24(6):715–733. doi: 10.1091/mbc.E12-07-0537

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Johnson et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 6: — Assembly of sorting-motif and serine mutants of Cx43 into gap junctions in knockdown cells. (A) WT-Cx43 or the indicated mutants were knocked in to Bx43KD and CP43KD cells, and lysates were analyzed for Cx43 expression by Western blotting. Note that, compared with parental cells expressing endogenous Cx43 (Endo-Cx43), the knocked-in WT-Cx43 and Cx43 mutants are robustly expressed. No detectable expression is seen in CP43KD and Bx43KD cells. (B) Bx43KD and CP43KD cells expressing the knocked-in WT-Cx43 or Cx43-mutants were immunostained for Cx43. Note that only the sorting motif mutant YA/VD-Cx43 (YA/VD) assembles into gap junctions.