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. 2013 Mar 15;24(6):748–756. doi: 10.1091/mbc.E11-08-0740

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© 2013 Iwasaki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Phosphorylation of MYPT1 during apoptosis. HeLa cells were treated with DMSO, TNF/CHX, or TNF/CHX/zVAD-FMK plus Y-27632 for 3 h. Phosphorylation of endogenous MYPT1 was determined using the indicated antibody (pT696, anti–phospho-Thr-696; pT853, anti–phospho-Thr-853; or pS903, anti–phospho-Ser-903 antibody). The full-length and caspase-3–cleaved MYPT1s are indicated using filled and open arrowheads, respectively.