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. 2013 Mar 15;24(6):748–756. doi: 10.1091/mbc.E11-08-0740

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© 2013 Iwasaki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Localization of caspase-3–cleaved MYPT1. HeLa cells transiently transfected with V5-MYPT1-WT (A–D), V5-MYPT1-D884A (E–H), V5-MYPT1-(1-884) (I–L), V5-MYPT1-T696DT853D (M and N), and V5-MYPT1-T696DT853D-(1-884) (O and P) were doubly stained with anti-V5 antibody and either with anti–myosin heavy chain IIA antibody or with phalloidin. Localization of MYPT1 at the cortex region is indicated by white arrowheads. Scale bar: 30 μm. Graph shows percentage of the cells in which V5-MYPT1 is localized at stress fibers. Results shown are mean ± SD of three independent experiments.