FIGURE 10:

Arf1, Pik1, and Sac1 modulate adaptor localization during glucose starvation. (A) Brefeldin A induces adaptor redistribution only during glucose starvation. erg6∆ cells expressing Gga2-GFP or Ent5-mCherry were imaged before or after 2 h of glucose starvation. Cells were treated with DMSO (control) or 150 μM brefeldin A for 5 min before imaging. (B) Adaptors show increased dependence on Pik1 during glucose starvation. pik1-83 cells expressing Gga2-mRFP or Ent5-GFP were imaged before or after 2 h of glucose starvation at the permissive temperature (25°C) or after a 30-min shift to nonpermissive (38°C) temperature. For temperature-shifted starved cells, cells were first starved for 1.5 h and then shifted to the nonpermissive temperature. (C) Gga2 but not Ent5 requires Sac1 for rapid redistribution during acute starvation. Wild-type and sac1∆ cells expressing Gga2-GFP or Ent5-GFP were imaged before or after 2 h of glucose starvation. Scale bar, 5 μm.