FIGURE 6:

ATP is sufficient to recruit adaptors in permeabilized cells. (A) Cells expressing Gga2-GFP or Ent5-GFP from the endogenous loci were permeabilized and incubated with or without indicated nucleotides for 5 min before imaging. (B) Ent5 is recruited to Sec7 containing organelles upon addition of ATP. Cells expressing Ent5-GFP and Sec7-mcherry were permeabilized and incubated with or without ATP. Cells were fixed, mounted for TIRF microscopy, and imaged. (C) Snf1 is not required for adaptor localization in the presence of exogenous ATP. Cells lacking Snf1 and expressing Gga2-GFP or Ent5-GFP from the endogenous loci were permeabilized and imaged with or without addition of exogenous ATP. Scale bar, 5 μm.