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. 2013 Mar 15;24(6):832–847. doi: 10.1091/mbc.E12-10-0750

FIGURE 7:

FIGURE 7:

ATP and GTP both contribute to adaptor recruitment. (A, B) Right, addition of GTP reduces the amount of ATP required to induce adaptor localization in permeabilized cells. Cells were prepared as in Figure 6 and incubated with indicated amounts of nucleotides for 5 min before imaging. Left, quantification of adaptor localization in different conditions. Cells were classified as having no puncta, only small or dim puncta, or large puncta. Scale bar, 5 µm. Charts show representative data from one of three replicate experiments. n > 50 cells. (C) GTP-γ-S reduces the amount of ATP required to induce adaptor localization.