TABLE 1:
Strains and plasmids.
| Strain or plasmid | Description | Source |
|---|---|---|
| BY4741 | mat a his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 | Invitrogen |
| BY4742 | mat α his3Δ1 leu2Δ0 ura3D0 lys2Δ0 | Invitrogen |
| DLY003 | BY4742 ENT5-GFP(S65T)-HIS3MX | Aoh et al. (2011) |
| DLY004 | BY4742 GGA2-GFP(S65T)-KANMX6 | Aoh et al. (2011) |
| DLY066 | BY4742 APL4-GFP(S65T)-KANMX6 | This study |
| Y1375 | Mat a ade2-1 can1-100 his3-11,15 leu2-3112 trp1-1 ura3-1 tpk2::KAN tpk3::TRP1 tpk1-M164G | Stephan et al. (2009) |
| DLY37 | BY4741 SEC7-MChERRY::HisMX ENT5-GFP::KANMX6 | This study |
| DLY067 | BY4742 reg1∆::KANMX4 | Invitrogen |
| DLY068 | BY4742 reg1∆::KANMX4 ENT5-GFP(S65T)-KANMX6 | This study |
| DLY070 | BY4742 snf1∆::HIS3MX | This study |
| DLY071 | BY4741 snf1∆::HIS3MX ENT5-GFP(S65T)-KANMX6 | This study |
| DLY072 | BY4742 snf1∆::HIS3MX GGA2-GFP (S65T)-KANMX6 | This study |
| DLY080 | Mat a/α his3∆1/his3∆1 leu2∆0/leu2∆0 met15∆0/met15∆0 LYS2/lys2∆0 ura3∆0/ura3∆0 GGA2-MCHERRY-KANMX/GGA2-MCHERRY-KANMX ARF1-GFP(S65T)-HIS3MX | This study |
| DLY100 | BY4742 gal83∆::KANMX4 | Invitrogen |
| DLY101 | BY4742 sak1∆::KANMX4 | Invitrogen |
| DLY102 | BY4742 Snf1-myc::His3MX::URA | This study |
| QAY632 | BY4742 ENT5-GFP(S65T)-KANMX6, KEX2-MCHERRY-HIS3MX | This study |
| QAY667 | BY4741 GGA2-GFP(S65T)-KANMX6 | This study |
| QAY768 | BY4741 SEC7-MCHERRY::HIS33MX APL4-GFP::KAMMX6 | This study |
| QAY769 | BY4742 SEC7-MCHERRY::HIS33MX GGA2-GFP::KAMMX6 | This study |
| QAY922 | BY4742 bmh1Δ-KANMX6 ENT5-GFP(S65T)-HIS3MX | This study |
| QAY1170 | Mat a/α his3∆1/his3∆1 leu2∆0/leu2∆0 met15∆0/MET15 LYS2/lys2∆0 ura3∆0/ura3∆0 ENT5-MCHERRY-KANMX/ENT5-MCHERRY-KANMX ARF1-GFP(S65T)-HIS3MX/ARF1 | This study |
| QAY1241 | BY4842 erg6Δ::KANMX GGA2-GFP(S65T)-HIS3MX | This study |
| QAY1244 | Mat α his3Δ1 leu2Δ0 ura3Δ0 met15Δ0erg6Δ::KANMX ENT5-MCHERRY-HIS3MX | This study |
| SEY6210 | Mat α ura3-52 lys2-801 ∆his3-200 leu2-3112, trp1-Δ901 | Robinson et al. (1988) |
| GPY404 | Mat a ura3-52 lys2-801 ∆his3-200 leu2-3112, trp1-Δ901 | Daboussi et al. (2012) |
| CTY244 | Mat a ura3-52 lys2-801 ∆his3-200 sac1∆1-354::HIS3 | Liu et al. (2008) |
| DLY 074 | CTY244 GGA2-GFP (S65T)-HIS3MX::URA | This study |
| DLY 075 | CTY244 ENT5-GFP(S65T)-HIS3MX::URA | This study |
| MDY655 | SEY6210 GGA2-GFP(S65T)-His3Mx::URA3 | Hung et al. (2012) |
| MDY399 | SEY6210 ENT5-GFP(S65T)-His3Mx::URA3 | Hung et al. (2012) |
| GPY4941 | GPY404 GGA2-mRFP::KANMX6 ENT5-GFP(S65T)::KANMX6 pik1Δ::HIS3 pRS314-pik1-83ts (TRP1 CEN6 pik1-83ts) | Daboussi et al. (2012) |
| GPY4940 | GPY404 GGA2-mRFP::KANMX6 ENT3-GFP(S65T)::KANMX6 pik1Δ::HIS3 pRS314-pik1-83ts (TRP1 CEN6 pik1-83ts) | Daboussi et al. (2012) |
| Y1375 | Mat a ade2-1 can1-100 his3-11,15 leu2-3112 trp1-1 ura3-1 tpk2::KAN tpk3::TRP1 tpk1-M164G | Stephan et al. (2009) |
| pCW26 | GFP-GOLPH3 | Wood et al. (2009) |
| pQA76 | pRS316-GFP-Pik1 | This study |