Figure 6.

NGF regulates the axonal proteins levels of Arp2, cortactin, and WAVE1 through PI3K signaling. A, Treatment with PI3Kpep, relative to the control PI3KpepAla, increased axonal levels of cortactin and WAVE1, but not Arp2. The effect of PI3Kpep on WAVE1 levels was strong and the y-axis is presented with a break to cover the range of fold differences between groups in the same graph. The number of axons analyzed is shown in the bars. Groups were compared using the Mann–Whitney test. B, Inhibition of PI3K signaling using LY294002 (LY; 10 μm, cotreatment) blocked the NGF-induced increases in the axonal levels of cortactin, WAVE1, and Arp2 (p value shown in bars). LY did not affect the baseline levels of proteins. Number of axons analyzed are shown in the bars. Within protein, the datasets were analyzed using the Kruskal–Wallis ANOVA with Dunn's post hoc tests.