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. 2013 Mar 13;8(3):e59233. doi: 10.1371/journal.pone.0059233

Figure 2. Atorvastatin treatment increased GTP loading and translocation of small GTPases from cell membrane to cytosol.

Figure 2

(A) HUVECs were treated with atorvastatin (10 µM) for the duration indicated. The whole cell lysates were harvested, and Rho, Rac and Cdc 42 GTP-loading assayed as described in Methods. (B) HUVECs were treated with atorvastatin (10 µM) overnight, the cells collected, and the small-GTPase GTP-loading of cytosol and cell membranes fractions prepared as described in Methods. Equal amounts of cell membrane and cytosolic protein were suspended in Laemmli buffer, and the GTPase loading determined by Western blotting using Rho, Rac and Cdc42 antibodies. Data in shown in graphs represent the means ± S.E. for 4 samples at each timepoint, * p<0.05 vs control; #P<0.01 vs control.