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. 2013 Mar 13;8(3):e59200. doi: 10.1371/journal.pone.0059200

Figure 1. Effect of adding cell lysate on Cr(VI) reductase activity of purified enzymes.

Figure 1

Reactions contained 150 μM Cr(VI), 500 µM NADH, and 0.03 mg/ml enzyme in a total volume of 100 μl made up with either 100%, 67%, 33% or 0% E. coli cell lysate as indicated in the key (the remaining volume being made up by 50 mM Tris-Cl buffer pH 7.0). Reactions were stopped at 5 min (NemA_Ec) or 30 min (NfsA_Kp and AzoR_Ec) by addition of 1,5-diphenylcarbazide color developing solution, and the amount of Cr(VI) reduced was calculated. Data are the mean of two independent replicates, and error bars indicate ±1 standard deviation.