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. 2013 Mar 13;8(3):e59200. doi: 10.1371/journal.pone.0059200

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid Description Source
E. coli
BL21 F-, ompT, hsdS B (rB- mB-), gal, dcm Novagen
BL21(DE3) F-, ompT, hsdS B (rB- mB-), gal, dcm (DE3) Novagen
SOS-R1 Strain W3110 F- lac-6(del)λΦ(sfiA::lacZ), nfsA, nfsB [23]
Plasmids
pUCX pUC19 derivative containing lacI, tac promoter, lac operator, RBS region and rrnB terminator from pMMB67EH. ApR. [23]
pET28a+ His6-tag expression vector. KmR. Novagen
pMCS69 pBBR1MCS derivative containing genes phaA and phaB of R. eutropha colinear to lac promoter, CpR. [43]
pET-14b:PhaC pET-14b derivative containing gene phaC of R. eutropha colinear to T7 promoter, ApR. [44]
pET-14b:PhaC-L-NemA_Ec pET14b derivative containing gene nemA of E. coli fused to the 3′ end of phaC by a linker sequence. This study