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Dichroic spectra of 4 µM Tel 26 in the presence of increasing concentrations of (K34)2Ni(II) at 45 °C (Panel A) and thermal denaturation profiles of the same oligonucleotide (Panel B) recorded at 290 nm in the absence (solid line) or in the presence of (K34)2Ni(II) at 2∶1 molar ratio (dotted line) in 10 mM Tris, 20 mM KCl at pH 7.5.
