Table 2. Main mechanisms controlling mRNA concentrations.
Compared cultures | Mainly transcriptional control ρD ≤0 | Shared control 0<ρD<1 | Mainly degradation control ρD ≥1 | ||||
Number of genes | Percentage of the total | Number of genes | Percentage of the total | Number of genes | Percentage of the total | ||
Chemostat | 0.80 vs. 0.51 h−1 | 479 | 98% | 4 | 1% | 4 | 1% |
0.51 vs. 0.11 h−1 | 454 | 84% | 75 | 14% | 14 | 3% | |
Batch | Exponential phase (0.8 h−1) vs. deceleration (0.38 h−1) | 452 | 72% | 109 | 17% | 67 | 11% |
Deceleration (0.38 h−1) vs. stationary (0.04 h−1) | 490 | 77% | 66 | 10% | 77 | 12% |
vs. indicates the growth conditions considered for the calculation of the regulation coefficients. Chemostat and batch indicate that RNA half-lives were determined in continuous or discontinuous cultures, respectively. In chemostat cultures, cells were at a steady state, with growth limited by the isoleucine concentrations. In batch cultures, isoleucine in the medium was progressively consumed (until starvation was reached) and the cells were in a dynamic process of adaptation.