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. 2013 Mar 13;8(3):e57959. doi: 10.1371/journal.pone.0057959

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© 2013 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The 12 purified proteins were probed with rabbit immune sera against live spores of B.anthracis vaccine strain A16R. The upper panel shows the SDS-PAGE maps of the 12 purified proteins, the middle panel is the western blotting assay with rabbit immune sera against B.anthracis live spores and the lower panel is the western blotting assay with the unimmunized rabbit sera. M:unstained protein molecular weight marker (Cwbio, China). (B) Western blotting assays for A16R whole cell proteins using immune sera from mice vaccinated with the 12 expressed proteins. A16R: whole cell lysate proteins of B. anthracis vaccine strain A16R; control: A16R whole cell proteins were probed with unimmunized mice sera. M: Unstained protein molecular weight marker (Fermentas).