Table 1. Immunogenic proteins identified in this study.

Vegetative immunoreactive spots	Spore immunoreactive spots	Accession No.	gene	Protein description	MWa	pIb	Note
r028	-	BA0080	-	Negative regulator of genetic competence clpC/mecB	90532	6.06	[45]
r161	r189	BA0108	tuf	elongation factor Tu	43026	4.93	[29], [41], [45]
r099\r100\r101	r196	BA0267	groEL	chaperonin GroEL	57396	4.79	[29], [41], [45]
r066\r073\r096	-	BA0309	-	1-pyrroline-5-carboxylase dehydrogenase	56418	5.43	[45]
r094\r095	-	BA0345	ahpC	alkyl hydroperoxide reductase, subunitC	20878	4.81	[45]
r012\r015\r016\r018\r030\r031\r034\r035\r037\r048\r052\r063\r075\r142	-	BA0887	eag	S-layer protein EA1	91307	5.7	[26], [27], [28], [29], [30], [31], [36], [41], [45]
r007		BA1300	potD	putrescine-binding protein	39552	5.97	[29]
r049		BA1419	ilvC-1	Ketol-acid reductoisomerase	36784	5.17	[25]
r044	r181	BA3964	tsf	translation elongation factor Ts	32529	5.25	[29], [41]
-	r187	BA4184	pdhA	Pyruvate dehydrogenase E1 component subunit alpha	41415	5.52	[41]
r001\r002	r176	BA4499	sodA-1	Superoxide dismutase	22650	5.3	[26]
r097\r098	r198	BA4539	dnaK	molecular chaperone DnaK	65784	4.65	[29], [45]
r129	-	BA4705	tiG	trigger factor	47185	4.53	[41]
r132\r133	-	BA5364	eno	enolase	46446	4.66	[37], [41], [42], [45]
r009	r183\r184	BA5369	gap-2	glyceraldehyde-3-phosphate dehydrogenase	35974	5.37	[37], [41]
r005	-	BA5565	-	hypothetical protein BA5565	22274	5.45	[25]
r081\r147	-	BA5580	fba-2	fructose bisphosphate aldolase	30825	5.06	[37]
r151	-	BA3338	-	S-layer protein, putative	40427	5.84	[26], [45]
r054	-	BA4873	ald-2	Alanine dehydrogenase	40141	5.22	[45]
-	r178	BA1021	-	hypothetical protein BALH_0915	31558	6.01	[41]
r006	-	BA5588	-	3-hydroxybutyryl-CoA dehydrogenase	31533	5.45	[41]
r024	-	BA2958	-	3-deoxy-7-phosphoheptulonate synthase	40115	5.65	[41]
r154	-	BA1998	nadE	NH(3)-dependent NAD(+) synthetase	30083	4.94	[41]
r014	-	BA5061	-	hypothetical protein	25273	5.8	[41]
-	r205\r206	BA5017	metK	S-adenosylmethionine synthetase	43464	5.2	New
-	r203	BA5147	glyS	glycyl-tRNA synthetase	53497	5.09	New
-	r179	BA1232	fabI	enoyl-(acyl carrier protein) reductase	27894	5.45	New
r153	-	BA0347	-	NH(3)-dependent NAD(+) synthetase	38454	4.99	New
r149	-	BA4861	pepQ-2	proline dipeptidase;Peptidase M24, methionine aminopeptidase	40862	5.13	New
r148	-	BA1497	resD	DNA-binding response regulator ResD;Signal transduction response regulator, receiver domain;CheY-like superfamily	27514	5.08	New
r103	-	BA0344	ahpF	alkyl hydroperoxide reductase, F subunit	55061	4.93	New
r090	-	BA2013	dpS	general stress protein;DNA-binding protein Dps;Ferritin-like protein, Dps type	16638	4.79	New
r089	-	BA5290	-	general stress protein 20U;DNA-binding protein Dps;Ferritin-like protein, Dps type	16899	4.76	New
r087	-	BA0100	rplL	50S ribosomal protein L7/L12	12510	4.71	New
r072	-	BA1555	dapB	dihydrodipicolinate reductase	29294	5.36	New
r070	-	BA1536	-	nucleoside diphosphate kinase	16590	5.24	New
r069	-	BA0346	-	5-Methylthioribose kinas	47360	5.38	New
r067	-	BA2354	mmsA-1	methylmalonic acid semialdehyde dehydrogenase	53169	5.44	New
r065	-	BA1969	thrC	threonine synthase	37723	5.32	New
r057	-	BA4274	nagA	N-acetylglucosamine-6-phosphate deacetylase	41642	5.26	New
r055	-	BA4888	ackA	acetate/propionate kinase	43234	5.25	New
r050\r135	-	BA0079	-	arginine or creatine kinase;ATP guanido phosphotransferase	40238	5.24	New
r038	-	BA4385	bfmbC	dihydrolipoamide dehydrogenase	50914	5.53	New
r036	-	BA0550	prkA	probable serine protein kinase	73341	5.65	New
r019	-	BA1511	gdhA	glutamate/phenylalanine/leucine/valine dehydrogenase	47748	5.85	New
r017\r136\r150	-	BA0509	pfl	formate acetyltransferase	84712	5.65	New
r013	-	BA2551	-	enoyl-CoA hydratase	28559	5.69	New
r011	-	BA0894	-	enoyl-CoA hydratase	28746	5.66	New
r008	-	BA4839	citZ	Citrate synthase	41928	5.36	New
r003	-	BA4194	-	2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase	25745	5.42	New

Note: ^a:Molecular Weight

^b:Isoelectric Point