Table 1. New microsatellite primers for the leatherback turtle, Dermochelys coriacea. Ta - Annealing temperature; Repeat motif; Range of Fragment size (in base-pairs).
Locus | Primer sequence (5'-3') | Ta (°C) | Repeat motif | Range of Fragment size (bp) | A (n = 215) | AR (n = 215) |
Dc003 | F- 5'-AAGCCCTCAGCATTTGAAGT-3' | 60 | (ATCT)12 | 170–218 | 12 | 8.78 |
R- 5'-CAATTTATCACAAAGATATCACC-3' | ||||||
Dc005 | F - 5'-GGCCTTTGATGCAAGTAGGA-3' | 54 | (GT)19 (TC)7 | 183–205 | 9 | 4.35 |
R - 5'-CAGGACTCACATAAAGTG-3' | ||||||
Dc008 | F - 5'-AATTGGATGACGAGCAGGAC-3' | 66 | (GT)20 | 184–220 | 13 | 10.64 |
R - 5'-GTCTCTCGCTGCCCACTGCTC-3' | ||||||
Dc013 | F- 5'-ACCGGTGGCACCTTAGAGAC-3' | 66 | (AC)13 | 153–167 | 6 | 4.44 |
R- 5'-CCAACAGAAGTATCACATCATGTTCTC-3' |
A–Total number of alleles; AR–Allelic richness;