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. 2013 Mar 13;8(3):e57380. doi: 10.1371/journal.pone.0057380

Figure 7. Cerebral Synaptosomal Purinergic Receptors were Downregulated in the MIA Model and Restored to Normal by Antipurinergic Therapy.

Figure 7

(A) Western Analysis of Metabotropic P2Y2 and Ionotropic P2X7 receptors. Each lane contains the synaptosomes from 2–3 males isolated at 16-weeks of age (n = 4–5 per group). (B) P2Y2 receptor expression was decreased by over 50% by gestational poly(IC) exposure and normalized by suramin treatment (Sal-Sal = 100+/−7.3%; Sal-Sur = 62+/−4.6%; PIC-Sal = 48+/−4.7%; PIC-Sur = 84+/−4.7%; one-way ANOVA F(3,12) = 18.1; p<0.0001; n = 4–5 males per group). (C) P2X7 receptor expression was decreased over 50% by gestational poly(IC) exposure and normalized by suramin treatment (Sal-Sal = 100+/−2.2%; Sal-Sur = 39+/−12%; PIC-Sal = 47+/−0.5%; PIC-Sur = 81+/−1.5%; one-way ANOVA F(3,12) = 23.2; p<0.0001; n = 4–5 males per group). Post-synaptic density 95 (PSD95) protein was used as a loading control. Values are expressed as mean +/− SEM.