Table 2. Putative D. bruxellensis glucose transporter genes expressed under conditions of oxygen and glucose limitation in continuous culture and corresponding S. cerevisiae and K. pastoris homologues.
D. bruxellensis gene | S. cerevisiae homologs | K. pastoris homologs | Gene function | RPKMa |
AWRI1499_3136 | HXT6 (4.0×10−146), HXT7 (4.0×10−146) | XP_002490706 (3.0×10−156) | Glucose transporter | 3371.55 |
AWRI1499_3135 | GAL2 (0), HXT6 (0), HXT7 (0) | XP_002490706 (0) | Hexose transporter | 2234.21 |
AWRI1499_4734 | HXT2 (6.0×10−38) | XP_002489344 (1.0×10−62) | Hexose transporter | 268.06 |
AWRI1499_2919 | HXT13 (2.0×10−20), HXT17 (2.0×10−20) | XP_002492232 (7.0×10−72) | High-affinity glucose transporter | 70.72 |
Numbers in parentheses show the Blast E-values of sequence comparison between the D. bruxellensis gene and the respective homologue.
RPKM is a measure of gene expression level expressed as number of reads per 1-K base pairs and million mapped reads.