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. 2013 Mar 13;8(3):e58413. doi: 10.1371/journal.pone.0058413

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© 2013 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Flow cytometry analysis for VEGFR2 expression in huh7 cells transiently transfected with empty vector and Klotho plasmid, respectively. (B) Caspase 3/7 activity assay after detachment for 48 hours, (C) Colony formation assay, and (D) TUNEL assay after 48 hours detachment for Huh7 cells stably transfected with empty vector and Klotho plasmid, respectively, without or with DMSO or VEGFR2 inhibitor Axitinib, and isotype IgG or anti-VEGFR2 blocking antibody treatment. The graphic profiles represent the mean of three independent replicates in each group with standard error bars and were statistically analyzed with a t test (*, P<0.05).