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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Mol Cancer Ther. 2013 Jan 16;12(3):326–338. doi: 10.1158/1535-7163.MCT-12-0901

Figure 2. YM-155 inhibits cell proliferation in malignant human glioma cell lines.

Figure 2

A, Glioma cells were incubated with varying concentrations of YM-155 for 72 hours; proliferation inhibition was assessed semiquantitatively by spectrophotometric measurement of MTS bioreduction. Points represent the mean of three measurements ± S.D. B, Glioma cells were seeded at 60% confluence, and allowed to attach overnight. Cell extracts were subjected to Western blotting analysis with the indicated antibodies. C, Glioma cells were treated with the indicated concentrations of YM-155 for 24 h. Cell extracts were subjected to Western blotting analysis with anti-survivin antibody. D, Glioma cells were grown as in B, and subjected to Western blotting analysis with the indicated antibodies. The blots were subsequently stripped and reprobed with total EGFR.