Fig. 1.

Increased leptin levels cause free radical and nitrosative stress in DIO-steatohepatitic mice. Lean control or high fat fed diet induced obese (DIO) mice were treated with either olive oil (vehicle) or CCl₄. A. Serum levels at 24 h post CCl₄ B. Liver homogenates from DIO, OB/OB, leptin supplemented and DIO mice injected with leptin antibody were subjected to immuno-spin trapping and anti-DMPO immunoreactivity was measured using ELISA. C. Frozen liver slices were analyzed for 3-nitrotyrosine immunoreactivity. C. Relative fluorescence intensities of 3-nitrotyrosine immunoreactivity from mice livers (n=4). *P<0.05