Table 1.

Measured secondary structure in nuclear receptor NTD and or AF1 domains

Receptor	Conditions	Secondary structure elements					Method	Refs.
		α-helix	β-strand	β-turn	Bend	Coil
AR-AF1	Buffer	13	20	32		36	CD	176
	Buffer	16	24	17	19	24	FTIR	188
	TFE	40	15	20		25	CD	176
	TMAO	37	17	11	11	24	FTIR	188
	+ TFIIF	35	15	14	17	21	FTIR	188
AR-NTD	Buffer	14	27	24		34	CD	242
	TFE	58	12	9		22	CD	242
GR-AF1	Buffer	26.7	11.6	12.3	10	39.4	FTIR	243
	Buffer	26	13			61	CD	180
	+ TBP	42.1	11.8	12.4	9.3	24.4	FTIR	243
	P-serine	45	19			36	CD	180
MR-AF1a	Buffer	11	29	25		36	CD	135
	TFE	63	5	10		22	CD	135
MR-MD	Buffer	13	22	24		40	CD	135
	TFE	66	13	7		15	CD	135
MR-AF1b	Buffer	19	32	31		19	CD	135
	TFE	13	31	23		33	CD	135
MR-NTD	Buffer	20	21	24		35	CD	135
	TFE	79	2	3		15	CD	135
PR-AnDBD	Buffer	30					CD	97
	+ JPD2	58	11			17	CD	97
EcR-NTD	Buffer	8	31	23		89	CD	244
dHR38-NTD	Buffer	11.1	11.7	10.3		66.6	CD	245
	TFE	72.3	4.4	8.7		17.8	CD	245

JPD2, Jun-protein dimerization 2; P-serine, phosphorylated serine(s); TMAO, trimethylamine N oxide; EcR, ecdysone receptor; CD, circular dichroism; FTIR, Fourier transform infrared spectroscopy.