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. 2010 Jul;24(4):308–316. doi: 10.1016/S1674-8301(10)60043-7

Fig.3. IL-13 inhibits LPS-induced NF-κB nuclear translocation.

Fig.3

HMCs were pretreated for 30 min with different concentrations (0-100 ng/mL) of IL-13 followed by 1 h incubation with 10 µg/mL LPS. After these treatments, cytoplasmic protein (CP) and nuclear protein (NP) extracts were prepared and then assayed by western blot for p65 protein (A). Densitometry of the CP p65 (B) and NP p65 (C) immunoblot bands shown in A were expressed in arbitrary units as the ratio of the control. Values represent mean±SD (n = 3, **P < 0.01).