Fig. 3.

Immunostaining for TLR4 and podoplanin in the ICR and ICR-STZ mouse kidneys. The immunostained sections were re-stained by HE staining. The HE staining shows that the kidney tissue is collapsed by edema and renal tubules are expanding in the cortex of the STZ-injected ICR mouse kidney (ICR-STZ). In the ICR mouse kidney section, all glomeruli were immunostained by the antibody for the podocyte marker, podoplanin (PDPN), while there were no cells reacting with anti-TLR4. In the ICR-STZ mouse kidney sections, almost all podoplanin-positive glomeruli were immunostained by anti-TLR4 (arrowheads) whereas the Bowman capsules, distal and proximal tubules, collecting tubules, lymphatic vessels, and blood vessels outside the glomeruli, were not immunostained. Bar=100 µm.