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. 2013 Feb 25;168(6):1375–1387. doi: 10.1111/bph.12042

Figure 1.

Figure 1

Expression of hCCX-CKR does not influence expression of hCXCR3. Immunocytochemical stainings revealed that non-transfected HEK293 cells did not express hCXCR3 (A) nor hCCX-CKR (B), whereas positive labelling was observed in hCXCR3 (D), hCCX-CKR (F), and hCXCR3/hCCX-CKR (H) stably transfected HEK293 cells. Cells stained in the absence of primary antibodies or in presence of an isotype control antibody with irrelevant specificity were devoid of signals (C,E,G). The magnification used in panels A-H was 40X. (I) ELISA experiments showed no influence of hCCX-CKR co-expression on hCXCR3 protein levels when compared with single hCXCR3-expressing cells. No hCXCR3 protein expression was found in mock- or hCCX-CKR-transfected cells. Data represent the means ± SEM of three independent experiments. Statistical significance against mock transfected cells was tested by means of multiple comparison anova and Tukey's post hoc analysis. Asterisks indicate P ≤ 0.05.