Figure 5.

Negative ligand binding cooperativity occurs in cells expressing both hCCX-CKR and hCXCR3. [¹²⁵I]-CXCL10 (A-B), [¹²⁵I]-CXCL11 (C-D) and [¹²⁵I]-CCL19 (E-F) equilibrium binding was performed with HEK293T cells expressing hCXCR3 alone (A and C), hCCX-CKR alone (E) or both hCXCR3 and hCCX-CKR (B, D, F), in the absence or presence of unlabeled chemokines (100 nM) as indicated. Data are given as averages ± SEM of normalized specific binding from 2–5 independent experiments performed in triplicate. Statistical significance against vehicle samples was tested by means of multiple comparison ANOVA and Tukey's post hoc analysis. Asterisks indicate P ≤ 0.05.