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. 2013 Feb 25;168(6):1375–1387. doi: 10.1111/bph.12042

Figure 7.

Figure 7

Presence of hCCX-CKR is associated with suppression of chemotaxis towards CXCL10 in T cells (A) Silencing hCCX-CKR using vectors containing DNA encoding for short hairpin RNA targeting hCCX-CKR resulted in a down-regulation of hCCX-CKR expression by freshly isolated T cells as determined by flow cytometry. Data are representative for 4 different human donors. (B) Down-regulating hCCX-CKR rescued chemotaxis towards CXCL10. Data are representative for five different human donors and presented as mean ± SEM. *P < 0.05 (Mann-Whitney U-test). (C) Flow cytometric analysis showed successful transfection of PHA/IL-2-treated T cells with hCCX-CKR. The data represent one out of five separate experiments with different human donors. (D) Re-expressing hCCX-CKR in PHA/IL-2-treated T cells inhibited CXCR3-mediated chemotaxis towards CXCL10. The data are representative for 6 separate experiments using different human donors and are presented as mean ± SEM. *P < 0.05 (Mann-Whitney U-test).