Table 2.

Chemokine receptors tested for chemotaxis inhibition by hCCX-CKR

Receptor	Ligand (1 nM)	% of migration (control is set to 100%)	n	P-value
CCR2 CCR2+CCX-CKR	CCL13	125 ± 13 92 ± 6	3	0.069
CCR4 CCR4+CCX-CKR	CCL17	139 ± 5 111 ± 11	3	0.069
CCR5 CCR5+CCX-CKR	CCL4	143 ± 13 121 ± 6	3	0.15
CCR6 CCR6+CCX-CKR	CCL20	135 ± 10 96 ± 9	3	0.02
CCR7 CCR7+CCX-CKR	CCL21	125 ± 15 75 ± 9	3	0.023
CCR9 CCR9+CCX-CKR	CCL25	168 ± 15 94 ± 5	3	<0.001
CCR10 CCR10+CCX-CKR	CCL27	158 ± 15 84 ± 11	3	0.003
CXCR3 CXCR3+CCX-CKR	CXCL10	148 ± 9 82 ± 7	3	<0.001
CXCR4 CXCR4+CCX-CKR	CXCL12	154 ± 16 83 ± 10	3	0.009

HEK293T cells were either transfected with a chemokine receptor alone or with a combination of a chemokine receptor and CCX-CKR. Cells were then subjected to a chemotaxis experiment using the chemokine specific for the transfected receptor diluted in medium at a concentration of 1 nM. Control migration was done in presence of medium alone. In all control experiments, between 25 and 45 cells migrated towards the medium. Using these numbers, control migration was set to 100%. Data represent the mean and SEM of 3 independent experiments. ANOVA was performed to illustrate statistical differences in migration. P < 0.05 indicates that the chemotaxis mediated by the chemokine receptor towards its ligand was inhibited by the presence of CCX-CKR.