Figure 4.

Effect of ramosetron and ondansetron on the destruction of intestinal crypts induced by 5-FU in mice. Animals were administered 5-FU (50 mg·kg⁻¹, i.p.) once daily for 5 days (days 0 to 4) and killed 5 days after the onset of 5-FU treatment (Day 5). Ramosetron (RAM, 0.01, 0.03, and 0.1 mg·kg⁻¹) and ondansetron (OND, 5 mg·kg⁻¹) were administered p.o. twice daily for 5 days. Haematoxylin and eosin staining was performed (A, 500×), and the number of surviving crypts per millimetre (B) and surviving crypt cells per crypt (C) were measured by light microscopy. Data are presented as the means ± SEM from six mice. Significant differences at P < 0.05; *From control (C, vehicle alone); ^#from normal (N, 5-FU-untreated).