Figure 5.
Effect of ramosetron on apoptosis in the intestinal crypt induced by 5-FU in mice. Animals were administered 5-FU (50 mg·kg−1, i.p.) once daily and killed 1 and 3 days after the initiation of 5-FU treatment. Ramosetron (RAM, 0.1 mg·kg−1) was administered p.o. twice daily. Apoptosis was assessed using the TUNEL assay (A, 500×). The number of TUNEL-positive cells was counted in the intestinal crypt using a light microscope (B). Data are presented as the means ± SEM from six mice. Significant differences at P < 0.05; *from control (C, vehicle alone); #from normal (N, 5-FU-untreated).