Figure 8.
Effect of ramosetron on up-regulation of TNF-α, IL-1β, IL-6, IFN-γ mRNA expression in the small intestine induced by 5-FU in mice. Animals were administered 5-FU (50 mg·kg−1, i.p.) and killed 0.5, 1 and 3 days later. Ramosetron (RAM, 0.1 mg·kg−1) was administered p.o. twice daily. The expression of TNF-α, IL-1β and IL-6 was quantified by real-time RT-PCR. Expression levels of each mRNA were standardized to that of β-actin mRNA, and normalized to the mean value for day 0 or normal (5-FU-untreated) mice at each time point. (A) Changes in TNF-α, IL-1β, IL-6 and IFN-γ expression during the course of 5-FU treatment. Effect of ramosetron on the up-regulation of TNF-α (B), IL-1β (C), IL-6 (D), and IFN-γ (E) induced by 5-FU. Data are presented as the means ± SEM from six mice. Significant differences at P < 0.05; *from control (C, vehicle alone); #from normal (N, 5-FU-untreated).