Table 1.

Effects of various concentrations of CBDA on mean EC₅₀ and E_max values of 8-OH-DPAT for its stimulation of [³⁵S]GTPγS binding to rat brainstem membranes

Pretreatment	Mean EC50 (nM)	95% confidence limits (nM)	Mean Emax (%)	95% confidence limits (%)	n
Vehicle	5.0	1.6 and 15.0	29.1	24.6 and 33.7	7
0.01 nM CBDA	10.4	3.6 and 30.1	31.3	26.7 and 35.9	7
Vehicle	11.4	5.0 and 25.9	29.2	25.1 and 33.3	7
0.1 nM CBDA	43.9	21.9 and 88.1	45.1*	39.8 and 50.3	7
Vehicle	6.0	1.8 and 20.3	26.8	22.2 and 31.4	6
1.0 nM CBDA	15.5	4.0 and 59.7	45.1*	36.7 and 53.4	6
Vehicle	12.6	5.3 and 30.0	29.1	25.2 and 33.0	7
10 nM CBDA	43.7	21.1 and 90.2	46.8*	40.9 and 52.8	7
Vehicle	9.5	4.1 and 21.9	22.9	19.9 and 25.8	7
100 nM CBDA	26.3	13.4 and 51.6	37.1*	33.0 and 41.2	7
Vehicle	7.8	3.1 and 19.8	31.7	26.7 and 36.7	6
1000 nM CBDA	18.3	5.0 and 67.4	28.8	22.6 and 35.0	6

^*The 95% confidence limits of this mean value do not overlap with those of the mean value in the previous row, indicating it to be significantly greater than the mean value obtained from experiments with vehicle-treated membranes (P < 0.05). See also Figure 8.