Table 1. Polymorphisms selection and primer sets used for the amplification of LMP2/LMP7.
Gene | Position and substitution of the amino acid | PCR primers(forward/reverse) | Annealing temperature | Enzyme and fragment (bp) | SNP ID |
LMP2 | 60(GCA→ACA) Arg→His |
5′-GTGAACCGAGTGTTTGACAAGC-3′ 5′-GCCAGCAAGAGCCGAAAC AAG-3′ |
57°C | Hin6I(39 + 213) | rsl7587 |
LMP7 | 145(CAG→AAG) Gln→Lys |
5′-TCATGGCGCTACTAGATGTATG-3′ 5′-AACTCTTTGTCCT AACTTGC AC-3′ |
55°C | BsmI(146+ 205) | rs2071543 |