. 2011 Mar;25(2):148–154. doi: 10.1016/S1674-8301(11)60019-5

Table 1. Primer sequences for construction and sequencing of scFv antibody.

Primers	Sequence (5′ → 3′)
V_HF	CGCCATGGGATCCCTCGAGGTCCAGCTGCAGCAG
V_HR	GGAAGATCTAGAGGAACCACCTGCAGAGACAGTGACCAGAGTCC
V_LF	GGTGGTTCCTCTAGATCTTCCTCCTCTGGTGGCGGTGGCTCGGGCGGTGGT
	GGGGAGCTCGATATTTTGATGACCC
V_LR	GGAATTCCAGGACCGCCTCCTGGTTTGATTTCCAGTTTGGTCCC

Restriction sites Nco I and EcoR I are underlined. Boldface indicates the DNA sequences used to integrate an 18-residue linker.