Table 1. The sequences of primers used for real-time PCR.
Gene | Primer sequence | Product length |
HIF-1α | Forward 5′-TCTGGGTTGAAACTCAAGCAACTG- 3′ | 150 bp |
Reverse 5′- CAACCGGTTTAAGGACACATTCTG-3′ | ||
VEGF | Forward 5′-TCACAGGTACAGGGATGAGGACAC- 3′ | 72 bp |
Reverse 5′-CAAAGCACAGCAATGTCCTGAAG-3′ | ||
β-actin | Reverse 5′-CAAAGCACAGCAATGTCCTGAAG-3′ | 186 bp |
Reverse 5′-CTAAGTCATAGTCCGCCTAGAAGCA-3′ |