Table 1.
Summary of 2D DIGE analysis of liver extracts. Protein extracts were prepared from the livers of LPS-treated wild-type (WT) and GSNOR −/− knockout (KO) mice
| Fluorescent dye | No. of spots | Genotype/group | |
|---|---|---|---|
| 1 | Cy3 | 4111 | WT-1 |
| Cy5 | 4111 | KO-1 | |
| Cy2 | 4111 | Standard | |
| 2 | Cy3 | 4513 | WT-2 |
| Cy5 | 4513 | KO-2 | |
| Cy2 | 4513 | Standard | |
| 3 | Cy3 | 3920 | WT-3 |
| Cy5 | 3920 | KO-3 | |
| Cy2 | 3920 | Standard | |
| 4 | Cy3 | 4264 | KO-1 |
| Cy5 | 4264 | WT-2 | |
| Cy2 | 4264 | Standard | |
| 5 | Cy3 | 3979 | KO-2 |
| Cy5 | 3979 | WT-3 | |
| Cy2 | 3979 | Standard | |
| 6 | Cy3 | 4019 | KO-3 |
| Cy5 | 4019 | WT-1 | |
| Cy2 | 4019 | Standard | |
| 7 | Cy3 | 4047 | WT-1 |
| Cy5 | 4047 | KO-2 | |
| Cy2 | 4047 | Standard | |
| 8 | Cy3 | 3874 | WT-2 |
| Cy5 | 3874 | KO-3 | |
| Cy2 | 3874 | Standard | |
| 9 | Cy3 | 4378 | WT-3 |
| Cy5 | 4378 | KO-1 | |
| Cy2 | 4378 | Standard |
Internal standard (Standard) sample was prepared by pooling equal amounts of proteins from each extract. As shown, samples were labeled with the indicated fluorescent dyes and subjected to 2D DIGE as described in Section 2. This table also includes information on the number of protein spots detected on each 2D DIGE gel and genotype/group of the sample used for the 2D DIGE analysis.