Table 1. Apparent kinetic constants of Lex-Lex interaction between SLBs and liposomes*.
kon/M−1s−1 | koff/s−1 | Ka/M−1 | ||
---|---|---|---|---|
without cholesterol | 0 mM CaCl2 | (0.9 ± 0.2) × 103 (R2 = 0.99) | 0.40 ± 0.05 (R2 = 0.98) | 0.2 × 104 |
0.9 mM CaCl2 + 0.5 mM MgCl2 | (1.2 ± 0.5) × 103 (R2 = 0.98) | 0.40 ± 0.05 (R2 = 0.99) | 0.3 ×104 | |
10 mM CaCl2 | (1.2 ± 0.4) × 103 (R2 = 0.97) | (44 ± 5) · 10−3 (R2 = 0.98) | 2.7 × 104 | |
2 wt% cholesterol | 0 mM CaCl2 | (0. 9 ± 0.3) ×103 (R2 = 0.98) | 0.15 ± 0.02 (R2 = 0.95) | 0.6 × 104 |
0.9 mM CaCl2 + 0.5 mM MgCl2 | (3.0 ± 0.9) × 103 (R2 = 0.99) | 0.11 ± 0.01 (R2 = 0.97) | 2.7 × 104 | |
10 mM CaCl2 | (3.2 ± 1.0) × 103 (R2 = 0.98) | (62 ± 5) · 10−3 (R2 = 0.98) | 5.2 × 104 |
*Kinetic constants are given as the mean and its standard deviation of a triplet of experiments. R2-values for the goodness of fitting are given in parenthesis.