Table 1.
Percentage of segments with free energy below −40 kcal/mol
| Region | Non-fragile | FRA10G | FRA10C | FRA10D | FRA10A | FRA10B/E | FRA10F |
|---|---|---|---|---|---|---|---|
| Segments ΔG > −40 kcal/mola | 471 257 (91.1%) | 56 047 (87.8%) | 114 086 (96.7%) | 22 991 (80.2%) | 63 094 (93.7%) | 18 724 (93.6%) | 48 948 (87.9%) |
| Segments ΔG < −40 kcal/molb | 46 044 (8.9%) | 7820 (12.2%) | 3914 (3.3%) | 5677 (19.8%) | 4241 (6.3%) | 1277 (6.4%) | 6717 (12.1%) |
| P-value* | – | 2.34E−166 | 0 | 0 | 1.21E−133 | 7.04E−35 | 4.67E−133 |
aTotal number of segments with a free-energy value higher or lower than −40 kcal/mol, with the percentage of the total in parentheses.
bFragile sites highlighted in gray have a significantly greater proportion of segments with a free-energy value less than −40 kcal/mol compared with non-fragile DNA.
*P-value calculated by comparing each fragile site with non-fragile DNA using a chi-square test. A P-value of 0 represents a P < 1E−200.