Table 2.

Distribution of proportion of segments per section with free energy below −40 kcal/mol

Region	Chr 10	Non-fragile	FRA10G	FRA10C	FRA10D	FRA10A	FRA10B/E	FRA10F
Number of sections	17 403	10 334	1272	2360	574	1349	401	1113
Mean	0.087	0.089	0.122	0.033	0.198	0.063	0.064	0.121
SD	0.116	0.119	0.144	0.047	0.153	0.081	0.073	0.122
Mean difference from non-fragile	–	–	0.033	−0.056	0.109	−0.026	−0.025	0.032
Standard error	–	–	0.0042	0.0015	0.0065	0.0025	0.0038	0.0038
P-value*	–	–	2.27E−14	8.19E−279	1.17E−51	1.11E−23	1.39E−4	2.77E−15

*Overall, the mean difference among fragile and non-fragile sites analyzed by analysis of variance is highly significant (P = 7.7E−292). Subsequently, post-hoc Student's t-tests (two-tailed) were performed between each fragile site and non-fragile DNA. The P-values were adjusted for multiple comparisons by Bonferroni's correction.

**Fragile sites highlighted in gray have a significantly greater proportion of segments per section with a free-energy value less than −40 kcal/mol.