Table 14.1.
RNA helicases involved in ribosome biogenesis
| Gene designation (S. cerevisiae) |
Essential? | Helicase family |
Associated pre-rRNA processing defects | References | Human homolog |
|---|---|---|---|---|---|
| Small subunit (SSU) | |||||
| Dhr1 | Y | DEAH | Deficient in cleavage at A0, A1, and A2 | Colley et al. (2000), Granneman et al. (2006a) | DHX37 |
| Dhr2 | Y | DEAH | Deficient in cleavage at A0, A1, and A2 | Colley et al. (2000), Granneman et al. (2006a) | DHX32 |
| Dbp4 | Y | DEAD | Deficient in cleavage at A2, likely due to non-productive accumulation of U14 snoRNP on pre-rRNA transcript | Kos and Tollervey (2005) | DDX10 |
| Dbp8 | Y | DEAD | Deficient in cleavage at A0, A1, and A2 | Daugeron and Linder (2001), Granneman et al. (2006a) | DDX49 |
| Fal1 | Y | DEAD | Deficient in cleavage at A0, A1, and A2 | Kressler et al. (1997), Granneman et al. (2006a) | DDX48 |
| Rok1 | Y | DEAD | Deficient in cleavage at A0, A1, and A2, possibly due to non-productive accumulation of snR30 on pre-rRNA transcript | Venema et al. (1997), Bohnsack et al. (2008) | DDX52 |
| Rrp3 | Y | DEAD | Deficient in cleavage at A1 and A2 | O’Day et al. (1996), Granneman et al. (2006a) | DDX47 |
| Large subunit (LSU) | |||||
| Dbp3 | N | DEAD | Deficient in cleavage at A3 | Weaver et al. (1997) | N/A |
| Dbp6 | Y | DEAD | Reduction in 27S and 7S pre-rRNA levels | Kressler et al. (1998) | DDX51 |
| Dbp7 | N | DEAD | Reduction in 27S and 7S pre-rRNA levels | Daugeron and Linder (1998) | DDX31 |
| Dbp9 | Y | DEAD | Reduction in 27S and 7S pre-rRNA levels | Daugeron et al. (2001) | DDX56 |
| Dbp10 | Y | DEAD | Deficient in processing 27SB intermediates | Burger et al. (2000) | DDX54 |
| Drs1 | Y | DEAD | Reduction in mature 25S rRNA levels | Ripmaster et al. (1992), Bernstein et al. (2006) | DDX27 |
| Mak5 | Y | DEAD | Deficient in cleavage at A0, A1, and A2 | Bernstein et al. (2006) | DDX24 |
| Spb4 | Y | DEAD | Deficient in cleavage at A0, A1, A2, C1, and C2 | de la Cruz et al. (1998a,b), Bernstein et al. (2006) | DDX55 |
| Mtr4 | Y | Ski2-like | 7S pre-rRNA accumulation; deficient in cleavage at A0, A1, A2, and C2 | de la Cruz et al. (1998), Bernstein et al. (2006) | SKIL2 |
| Both subunits | |||||
| Has1 | Y | DEAH | Deficient in cleavage at A0, A1, and A2; Deficient in processing 27SA3 and 27SB intermediates; Accumulation of snoRNAs in pre-rRNA particles | Emery et al. (2004), Liang and Fournier (2006) | DDX18 |
| Prp43 | Y | DEAH | Deficient in processing 35S, 27S, and 20S intermediates | Combs et al. (2006), Leeds et al. (2006) | DHX15 |