Figure 5.

EWGP regulates PPARγ transcription activity and genes related to glucose and lipid metabolism. ((a) and (b)) The transcription activity of PPARα, γ. GAL4-DBD-LBD expression plasmids and a GAL4-responsive luciferase reporter were cotransfected into HEK293T cells for 24 h and treated with the PPARα and PPARγ agonists WY14643 and rosiglitazone (20 μM) and EWGP (50, 100, and 200 μg/mL) for another 24 h. The relative luciferase activities were measured by comparison to Renilla luciferase activities. The results represent three independent experiments, and data are presented as means ± SE. (c) The relative gene expression levels in the liver tissues from EWGP-treated and HF-fed mice in the preventive treatment. (d) The relative gene expression levels in the liver tissues from EWGP-treated and HF-fed mice in the therapeutic treatment. β-actin was used as an internal control for normalizing the mRNA levels. Data are presented as means ± SE for seven mice per group. *P < 0.05 versus HF group. (e) The relative gene expression levels in EWGP-treated and control HepG2 cells. β-actin was used as an internal control for normalizing the mRNA levels. Data are presented as means ± SE for six samples per group. *P < 0.05 versus control group.