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High glucose and pioglitazone induced NHE3 expression are mediated through EGFR phosphorylation. HK2 cells were incubated for 48 h with 5 mM glucose media (Control), high glucose (HG) ± Piog (10 μM) ± gefitinib (0.5 μM) and levels of NHE3 were determined by Western blotting. Representative images for NHE3 and actin bands are shown. Normalized results are expressed as mean ± SEM; n = 3. *P < 0.05 versus Control and δ P < 0.005 versus Control.
