Figure 8.

High-glucose-induced DNA binding of DIG-labeled NFκB. NFκB-DNA binding activity was significantly increased with high glucose. Pioglitazone significantly reduces high-glucose-induced NFκB binding activity. Gefitinib has a similar effect. HK2 cells were incubated for 72 hrs with 5 mM glucose media (Control), high glucose (HG) ± Piog (10  μM) ± gefitinib (0.5  μM). Nuclear extract preparation and EMSA are as described in Section 2. A representative image showing the shift band of NFκB is shown. Quantitative data are expressed as mean ± SEM; n = 3. *P < 0.05 versus control.