Figure 7.

Effect of NDGA on ((a), (c)) IAA or ((b), (d)) H₂O₂ induced increase of ((a), (b)) ROS levels and ((c), (d)) decrease of CD33 expression in MN. Cells were pretreated with vehicle or 20 μM NDGA for 12 h before the addition of 5 μM IAA or 1 mM H₂O₂ and incubated for 72 and 48 h, respectively. ROS levels detection and CD33 expression were determined by flow cytometry and expressed as index compared to control without treatment. Data are expressed as mean ± SEM; n = 5.  ⁺ P < 0.05 versus control (without IAA or H₂O₂) and  *P < 0.05 versus control (without NDGA).