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. 2012 Aug 9;12:91. doi: 10.1673/031.012.9101

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© 2012

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — A phylogenetic tree constructed from the amino acid sequences of CiHR3 and 15 related insect molt-regulating transcription factors aligned by ClustalW. A phylogenetic tree was constructed using MEGA4.0.2 software applying the method of Neighbor-Joining (NJ). The data set was subjected to 1000 bootstrap replicates. The bootstrap values higher than 50 are indicated at the corresponding branch. The bar represents 0.05 substitutions per 100 amino acids. Insect species and GenBank accessions are: Aedes aegypti, AAF36970; Galleria mellonella, AAA83401; Bombyx mori, NP_001037012; Plodia interpunctella, AAT08962; Mythimna separata, ACZ71480; Xestia cnigrum, ACZ71479; Tribolium castaneum, XP_974561; Apis mellifera, XP_392128; Blattella germanica, CAJ90623; Helicoverpa armigera, ACH86113; Drosophila melanogaster, AAA28461; Choristoneura fumiferana, AAC47163; Manduca sexta, CAA52654; Culex quinquefasciatus, XP_001865834; Anopheles gambiae, XP_319750; Chilo infuscatellus, JN835468. High quality figures are available online.