Figure 2.

Identification of dsRNA of CiHR3 and EGFP genes produced in bacteria. Total RNA was extracted from bacteria using bacterial RNA special extraction kit, and the RNA was treated with DNase I to remove the contamination of genomic DNA. The nucleic acid pellets were dissolved in RNase free H2O, loaded onto a 1.5% agarose/TBE gel, stained with nucleic acid stain and photographed, (a) RNA treated with DNase I treatment and (b) RNA without DNase I treatment. N, the lane with an RNA sample extracted from bacteria that were not treated with IPTG; I, the lane with an RNA sample extracted from bacteria that were treated with IPTG. The position of dsRNAs produced is marked with arrow heads. High quality figures are available online.