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. 2012 Jul 26;12:88. doi: 10.1673/031.012.8801

Figure 3.

Figure 3.

Degradation of apoLp-III from hemolymph extract of Galleria mellonella larvae by elastase B Pseudomonas aeruginosa in vitro. (A) Tricine SDS-PAGE and immunobloting with anti-Galleria mellonella apoLp-III antibodies. The samples containing hemolymph extract (20 µg of protein) and elastase B (0.05 µg (lane 2), 0.1 µg (lane 3), 0.2 µg (lane 4), 0.4 µg (lane 5)) were incubated at 37 °C for 60 min. The reactions were stopped by sample buffer addition. Lane 1 contained hemolymph extract only. (B) and (C) SDS-PAGE 2D electrophoresis. Hemolymph extract ( 100 µg of protein) and elastase B (0.6 µg) were incubated at 37 °C for 60 min. Then samples were loaded on pH 3 to l l isoelectric strips followed by Tricine SDS-polyacrylamide gel electrophoresis. For identification of apoLp-III anti-Golleria mellonella apoLp-III antibodies were used. (B) control sample of apoLp-III. (C) degradation products of apoLp-III are marked in circles. High quality figures are available online.