Table 1.

Method validation parameters for the quantitation of crypto-chlorogenic acid, isoquercetin, and astragalin by the proposed TLC-densitometric method.

Parameter	Results
	Crypto-chlorogenic acid	Isoquercetin	Astragalin
Linear range (ng/spot)	103–505	105–510	100–500
Calibration equation	y = −2589.922 + 15.123x	y = −884.153 + 16.772x	y = 511.081 + 20.486x
Correlation coefficient (r)	0.9961	0.9975	0.9968
LOQ (ng)	48.93	45.99	36.13
LOD (ng)	14.68	13.80	10.84

x is the amount of each standard in ng and y is the peak area at 340 nm.