Figure 3.

Binding and uptake of N8-GP in human monocyte-derived dendritic cells. (A-B) Monocytes were differentiated into dendritic cells by 5 days of culturing with granulocyte macrophage–colony-stimulating factor and interleukin 4 before incubating with ¹²⁵I–N8-GP or ¹²⁵I-FVIII for 24 hours at 4°C. Unspecific binding determined by adding 4 µM unlabeled N8-GP or FVIII was subtracted. (C) Internalization was assessed at 37°C for up to 3 hours using concentrations at K_d (ie, 110 nM ¹²⁵I–N8-GP and 70 nM ¹²⁵I-FVIII). Data are mean and standard deviation from 4 (A) or 3 (B-C) experiments.